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Nitrotyrosine Antikörper (Atto 488)

Dieses Anti-Nitrotyrosine-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Nitrotyrosine in WB, ELISA, IP, IHC, FACS, ICC, IF und AA. Geeignet für .
Produktnummer ABIN2486183

Kurzübersicht für Nitrotyrosine Antikörper (Atto 488) (ABIN2486183)

Target

Alle Nitrotyrosine Antikörper anzeigen
Nitrotyrosine

Reaktivität

Bitte anfragen

Wirt

  • 24
  • 21
  • 5
Maus

Klonalität

  • 26
  • 24
Monoklonal

Konjugat

  • 22
  • 5
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Nitrotyrosine Antikörper ist konjugiert mit Atto 488

Applikation

  • 48
  • 27
  • 24
  • 19
  • 17
  • 13
  • 13
  • 13
  • 9
  • 9
  • 8
  • 7
  • 4
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)

Klon

39B6
  • Spezifität

    Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

    Aufreinigung

    Protein G Purified

    Immunogen

    3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin

    Isotyp

    IgG2a
  • Applikationshinweise

    • WB (1:1400)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.

    Kommentare

    0.7 μg/ml of ABIN2486183 was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Conjugated antibodies should be stored at 4°C
  • Target

    Nitrotyrosine

    Substanzklasse

    Chemical

    Hintergrund

    Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).
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