TLR4 Antikörper (AA 420-435) (Biotin)

Produktnummer ABIN2485971

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TLR4 in WB, FACS, IHC, IF und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TLR4 Antikörper (AA 420-435) (Biotin) (ABIN2485971)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TLR4 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-TLR4 Antikörper (Biotin)

Bindungsspezifität: AA 420-435

Spezifität: Detects ~75-80 kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

Kreuzreaktivität: Human, Maus

Aufreinigung: Protein A Purified

Immunogen: Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4

Anwendungsinformationen

Applikationshinweise:

• WB (1:500)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Kommentare:

2 μg/ml of ABIN2485971 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: TLR4

Hintergrund: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa (6).

Gen-ID: 7099

NCBI Accession: NP_612564

UniProt: O00206

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine