Calnexin Antikörper (C-Term) (Atto 594)

Produktnummer ABIN2481633

Dieses Anti-Calnexin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Calnexin in WB, IHC, IF, IP, FACS und ICC. Geeignet für Hund.

Kurzübersicht für Calnexin Antikörper (C-Term) (Atto 594) (ABIN2481633)

Target: Calnexin (CANX)

Reaktivität: Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Calnexin Antikörper ist konjugiert mit Atto 594

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-Calnexin Antikörper (Atto 594)

Bindungsspezifität: C-Term

Spezifität: Detects the C-terminal domain of Calnexin ~90 kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.

Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Drosophila melanogaster, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Wachtel, Kaninchen, Ratte, Schaf, Xenopus laevis

Aufreinigung: Protein A Purified

Immunogen: Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• ICC/IF (1:100)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:2000 dilution of ABIN2481633 was sufficient for detection of Calnexin in 10 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu Calnexin

Target: Calnexin (CANX)

Andere Bezeichnung: Calnexin

Hintergrund: Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Gen-ID: 403908

NCBI Accession: NP_001003232

UniProt: P24643

Pathways: MAPK Signalweg, Thyroid Hormone Synthesis