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Growth Hormone 1 Antikörper

Der Maus Monoklonal Anti-Growth Hormone 1-Antikörper wurde für ELISA validiert und wurde unabhängig validiert. Er ist geeignet, Growth Hormone 1 in Proben von Human zu detektieren.
Produktnummer ABIN235517

Kurzübersicht für Growth Hormone 1 Antikörper (ABIN235517)

Target

Alle Growth Hormone 1 (GH1) Antikörper anzeigen
Growth Hormone 1 (GH1)

Reaktivität

  • 136
  • 20
  • 14
  • 11
  • 8
  • 8
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Human

Wirt

  • 86
  • 80
  • 2
  • 1
  • 1
Maus

Klonalität

  • 98
  • 72
Monoklonal

Konjugat

  • 102
  • 13
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Growth Hormone 1 Antikörper ist unkonjugiert

Applikation

  • 92
  • 92
  • 38
  • 38
  • 30
  • 22
  • 20
  • 13
  • 13
  • 13
  • 12
  • 9
  • 7
  • 6
  • 6
  • 5
  • 4
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA

Klon

090-10154
  • Kreuzreaktivität (Details)

    Cross-reactivity: Synthetic hGH (Somatonorm) 100 % Human Placental Lactogen <0.00 % Human Prolactin <0.00 %

    Produktmerkmale

    MAb to hGH, Monoclonal antibody to Human Growth Hormone (hGH)

    Aufreinigung

    Protein A Chromatography. Ascites

    Sterilität

    0.2 μm filtered

    Immunogen

    hGH isolated from human pituitary gland

    Isotyp

    IgG1
  • Applikationshinweise

    Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #102719 (Unfolding Profile)
    'Independent Validation' Siegel
    by
    NanoTemper Technologies
    No.
    #102719
    Datum
    05.11.2018
    Antigen
    GH1
    Chargennummer
    H0833.1
    Validierte Anwendung
    Unfolding Profile
    Positivkontrolle
    ABIN235517
    Negativkontrolle
    Bewertung

    Passed. ABIN235517 showed a clear unfolding profile with one detected unfolding event with a Ti at 76.3°C. This suggests that the protein is properly folded and functional.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN235517
    Sekundärantikörper
    Full Protocol
    • Dilute ABIN235517 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.33µM.
    • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Anmerkungen

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • Format

    Liquid

    Buffer

    10 mM Phosphate, pH 7.4 containing 150 mM Sodium chloride, 0.1 % Sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freeze/thaw cycles.
    Centrifuge product if not completely clear after standing at room temperature.
    Prepare working dilution only prior to immediate use.

    Lagerung

    4 °C/-80 °C

    Informationen zur Lagerung

    Short term (up to 7 days) store at 2-8 °C. Long term, aliquot and store at <-48 °C. If aliquoted for long term storage, fill volume should be equal to or greater than 50 % of the nominal fill volume of the vial used.

    Haltbarkeit

    1 week
  • Target

    Growth Hormone 1 (GH1)

    Andere Bezeichnung

    Growth Hormone (hGH)

    Pathways

    NF-kappaB Signalweg, JAK-STAT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
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