Growth Hormone 1 Antikörper

Produktnummer ABIN235517

Produktdetails anti-Growth Hormone 1 Antikörper

Target: Growth Hormone 1 (GH1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Growth Hormone 1 Antikörper ist unkonjugiert

Applikation: ELISA

Kreuzreaktivität (Details): Cross-reactivity: Synthetic hGH (Somatonorm) 100 % Human Placental Lactogen <0.00 % Human Prolactin <0.00 %

Produktmerkmale: MAb to hGH, Monoclonal antibody to Human Growth Hormone (hGH)

Aufreinigung: Protein A Chromatography. Ascites

Sterilität: 0.2 μm filtered

Immunogen: hGH isolated from human pituitary gland

Klon: 090-10154

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (UP)

Validierung #102719 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102719

Datum: 05.11.2018

Antigen: GH1

Chargennummer: H0833.1

Validierte Anwendung: Unfolding Profile

Positivkontrolle: ABIN235517

Bewertung:

Passed. ABIN235517 showed a clear unfolding profile with one detected unfolding event with a T_i at 76.3°C. This suggests that the protein is properly folded and functional.

Validierungsbilder

Unfolding profile of ABIN235517. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 76.3°C.

Protokoll

Primärantikörper: ABIN235517

Full Protocol:

• Dilute ABIN235517 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.33µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Anmerkungen:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Handhabung

Format: Liquid

Buffer: 10 mM Phosphate, pH 7.4 containing 150 mM Sodium chloride, 0.1 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Lagerung: 4 °C/-80 °C

Informationen zur Lagerung: Short term (up to 7 days) store at 2-8 °C. Long term, aliquot and store at <-48 °C. If aliquoted for long term storage, fill volume should be equal to or greater than 50 % of the nominal fill volume of the vial used.

Haltbarkeit: 1 week

Details zu Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Andere Bezeichnung: Growth Hormone (hGH) (GH1 Produkte)

Pathways: NF-kappaB Signalweg, JAK-STAT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process