Phospholipase C gamma 1 Antikörper

Produktnummer ABIN226218

Der Maus Monoklonal anti-Phospholipase C gamma 1 Antikörper (ABIN226218) detektiert spezifisch Phospholipase C gamma 1 in WB, ELISA, IP und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Rind (Kuh) und Kaninchen.

Kurzübersicht für Phospholipase C gamma 1 Antikörper (ABIN226218)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phospholipase C gamma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Phospholipase C gamma 1 Antikörper

Spezifität: Recognizes bovine PLC g-1 at 135kD. Reacts with some non-PLC g-1 SH-3 containing proteins, including nck (47kD). Species cross-reactivity: rabbit, rat, mouse and human.

Aufreinigung: Protein G purified

Immunogen: Purified soluble phospholipase C (PLC) g-1 from bovine brain.

Type of Immunogen: Purified protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations. Immunoprecipitation: 4 μg Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation. Immunocytochemistry: 5-10 μg/mL detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: dH2O, 40-50 % glycerol

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Andere Bezeichnung: PLCG1

Hintergrund: Name/Gene ID: PLCG1
Family: Phospholipase

Synonyms: PLCG1, Phospholipase C, gamma 1, Phospholipase C-148, Phosphoinositidase C, Phospholipase C-II, Inositoltrisphosphohydrolase, PLC-gamma-1, PLC1, PLC-148, PLC-II, PLC148, NCKAP3, Phospholipase C-gamma-1, PLCgamma1

Gen-ID: 5335

UniProt: P19174

Pathways: RTK Signalweg, WNT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling