LBP Antikörper

Produktnummer ABIN2191765

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch LBP in WB, Func, BR und IA. Er zeigt eine Reaktivität gegenüber Maus und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für LBP Antikörper (ABIN2191765)

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Functional Studies (Func), Blocking Reagent (BR), Immunoassay (IA)

Klon: M330-19

Produktdetails anti-LBP Antikörper

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Applikationshinweise: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts LBP to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-LBP Antikörper (ABIN2191765)

Le Roy, Di Padova, Adachi, Glauser, Calandra, Heumann: "Critical role of lipopolysaccharide-binding protein and CD14 in immune responses against gram-negative bacteria." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2759-65, (2001) (PubMed).

Le Roy, Di Padova, Tees, Lengacher, Landmann, Glauser, Calandra, Heumann et al.: "Monoclonal antibodies to murine lipopolysaccharide (LPS)-binding protein (LBP) protect mice from lethal endotoxemia by blocking either the binding of LPS to LBP or the presentation of LPS/LBP ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7454-60, (1999) (PubMed).

Details zu LBP

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Andere Bezeichnung: Lipopolysaccharide Binding Protein

Hintergrund: The monoclonal antibody M330-19 reacts highly specific with mouse natural and recombinant LBP. The antibody is a type I antibody blocking the LPS binding to LBP. LPS binding protein (LBP) is an approximately 60 kDa acute phase protein that is produced by hepatocytes. This protein strongly binds to LPS and has been shown to play an important role in the handling of LPS by the host. A number of functions of LBP have been reported. First, LBP transfers LPS to the LPS receptor CD14 on mononuclear phagocytes, leading to an 100-1,000-fold increased sensitivity of the cells to LPS. Furthermore, LBP can enhance the response of CD14 negative cells by acceleration of LPS binding to soluble CD14, a complex that stimulates these cells. Next, LBP transfers LPS into High Density Lipoprotein (HDL), which effectively neutralizes its biological potency. LBP was demonstrated to protect mice from septic shock caused by LPS or gram negative bacteria.

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process