LBP Antikörper

Produktnummer ABIN343173

Zitiert in 2+ Publikationen. Der Maus Monoklonal anti-LBP Antikörper (Klon biG33) (ABIN343173) detektiert spezifisch LBP in ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für LBP Antikörper (ABIN343173)

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LBP Antikörper ist unkonjugiert

Applikation: ELISA

Klon: BiG33

Produktdetails anti-LBP Antikörper

Spezifität: Mouse LBP. The antibody blocks LPS binding to membrane bound CD14.

Kreuzreaktivität: Human, Ratte (Rattus)

Aufreinigung: Protein G affinity column

Immunogen: native mouse LBP

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA ,LBP inhibition studies, Western Blot. inhibits binding of LPS to membrane bound CD14, (Inhibits LPS-binding (0.5 ug/ml) to CD14+CHO cells (2 x 106/ml) up to 1:1000, total inhibition 1:5 Binding titre at mouse LBP- ELISA: 1:50.000 Cross reacting with rat LBP

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with bidistilled water

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.17

Konservierungsmittel: Without preservative

Lagerung: -20 °C,-80 °C

Referenzen für anti-LBP Antikörper (ABIN343173)

Dziarski, Tapping, Tobias: "Binding of bacterial peptidoglycan to CD14." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8680-90, (1998) (PubMed).

Weidemann, Schletter, Dziarski, Kusumoto, Stelter, Rietschel, Flad, Ulmer: "Specific binding of soluble peptidoglycan and muramyldipeptide to CD14 on human monocytes." in: Infection and immunity, Vol. 65, Issue 3, pp. 858-64, (1997) (PubMed).

Details zu LBP

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Andere Bezeichnung: Lipopolysaccharide (LPS)-Binding Protein (LBP)

Hintergrund: Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes.

Molekulargewicht: ~58kDa

Gen-ID: 16803

UniProt: Q61805

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process