CDKN1B Antikörper (pSer10)

Produktnummer ABIN215394

Dieses Anti-CDKN1B-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CDKN1B in WB, IF und IP. Geeignet für Human, Maus und Ratte.

Kurzübersicht für CDKN1B Antikörper (pSer10) (ABIN215394)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDKN1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-CDKN1B Antikörper

Bindungsspezifität: pSer10

Spezifität: Recognizes p27 when it is phosphorylated at the Ser10 residue. Lysates of HEK 293T cells transfected with an expression plasmid encoding a p27 mutant where Ser10 was substituted with an alanine residue were used to confirm the antibody's specificity. Reactivity was confirmed with HEK293 cells expressing wild-type p27/KIP that is normally phosphorylated. NIH3T3 and MCF-7 cell lysates can also be used as positive controls. Species cross-reactivity: Human, rat and mouse.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IF (5 - 10 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1-3 μg/mL. Immunoprecipitation: 5-10 μg/IP reaction. Immunofluorescence: 5-10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Andere Bezeichnung: CDKN1B / p27 Kip1

Hintergrund: Name/Gene ID: CDKN1B

Synonyms: CDKN1B, KIP1, MEN1B, P27 Kip1, p27KIP1, MEN4, CDKN4

Gen-ID: 1027

UniProt: P46527

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie