CDK1 Antikörper

Produktnummer ABIN215066

Der Maus Monoklonal Anti-CDK1-Antikörper wurde für WB, IHC, IF, IP, IHC (p), IHC (fro) und Func validiert. Er ist geeignet, CDK1 in Proben von Human, Maus, Ratte, Huhn und Xenopus laevis zu detektieren.

Kurzübersicht für CDK1 Antikörper (ABIN215066)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reaktivität: Human, Maus, Ratte, Huhn, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Produktdetails anti-CDK1 Antikörper

Spezifität: Recognizes a 34kD protein cdk1, identified as cdk1/p34cdc2 protein kinase (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. It shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks). Human, mouse, rat, chicken, Xenopus.

Aufreinigung: Purified

Immunogen: Xenopus p34cdc2.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Func, IF, IHC, IHC-Fr, IHC-P, IP, WB (2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Immunoprecipitation (Native, denatured): 2 μg/mg protein lysate using Protein A. Immunohistochemistry (formalin/paraffin, frozen): 2-4 μg/mL for 30 minutes at RT. Paraffin sections require antigen retrieval. Western Blot: 2 μg/mL for 2 hours at RT. Positive control: HeLa or NIH3T3 cells, germinal center in a tonsil or reactive lymph node.

Kommentare:

Target Species of Antibody: Xenopus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDK1 / CDC2

Hintergrund: Name/Gene ID: CDK1
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR

Gen-ID: 983

UniProt: P06493

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA