E2F1 Antikörper (pSer364)
Kurzübersicht für E2F1 Antikörper (pSer364) (ABIN214450)
Target
Alle E2F1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- pSer364
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Spezifität
- Amino acids 360-369 of Human E2F-1.
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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Synthetic peptide from human E2F1.
Type of Immunogen: Synthetic peptide
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Applikationshinweise
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Approved: ELISA (1:20000 - 1:100000), IHC, IHC-P (10 μg/mL), WB (1:250 - 1:2000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- E2F1 (E2F Transcription Factor 1 (E2F1))
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Andere Bezeichnung
- E2F1
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Hintergrund
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Name/Gene ID: E2F1
Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3 -
Gen-ID
- 1869
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UniProt
- Q01094
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Pathways
- p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie
Target
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