Caspase 1 Antikörper (AA 371-390)

Produktnummer ABIN213734

Der Maus Monoklonal Anti-Caspase 1-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, Caspase 1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für Caspase 1 Antikörper (AA 371-390) (ABIN213734)

Target: Caspase 1 (CASP1)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caspase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 14F468

Produktdetails anti-Caspase 1 Antikörper

Bindungsspezifität: AA 371-390

Marke: IHC-plus™

Spezifität: Will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.

Aufreinigung: Protein G purified

Immunogen: A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTEof human caspase-1.

Type of Immunogen: Synthetic peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (20 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Caspase 1

Target: Caspase 1 (CASP1)

Andere Bezeichnung: CASP1 / Caspase 1

Hintergrund: Name/Gene ID: CASP1
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP1, Caspase-1, CASP-1, CASP1 nirs variant 1, Caspase 1, ICE, ICE-like protease, IL-1 beta-converting enzyme, IL-1BC, IL1BC, IL1BCE, p45, IL1B-convertase, Interleukin-1 beta convertase

Gen-ID: 834

UniProt: P29466

Pathways: Apoptose, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome