Paxillin Antikörper

Produktnummer ABIN212824

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Paxillin in WB, ELISA, IP und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Huhn und Rind (Kuh).

Kurzübersicht für Paxillin Antikörper (ABIN212824)

Target: Paxillin (PXN)

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Paxillin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Paxillin Antikörper

Spezifität: Recognizes human Paxillin at 68kD and a band around 50kD, which is most likely a degradation product. Species cross-reactivity: Human, mouse, rat, chicken and bovine. Other species not tested.

Aufreinigung: Protein G purified

Immunogen: GST fusion protein containing full-length human paxillin.

Type of Immunogen: Fusion protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects paxillin in RIPA lysates from 3T3 fibroblasts using 20 μg per lane for minigels. Immunoprecipitation: 4 μg per 500 μg of cell lysate. Immunocytochemistry:5-10 μg/mL (gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7 % paraformaldehyde for 5 minutes).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Paxillin

Target: Paxillin (PXN)

Andere Bezeichnung: PXN / Paxillin

Hintergrund: Name/Gene ID: PXN

Synonyms: PXN, Paxillin

Gen-ID: 5829

UniProt: P49023

Pathways: MAPK Signalweg, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling