Paxillin Antikörper

Produktnummer ABIN212824

Dieser Maus Monoklonal Antikörper detektiert spezifisch Paxillin in WB, ELISA, IP und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Huhn und Rind (Kuh).

Kurzübersicht für Paxillin Antikörper (ABIN212824)

Target: Paxillin (PXN)

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Paxillin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Paxillin Antikörper

Spezifität: Recognizes human Paxillin at 68kD and a band around 50kD, which is most likely a degradation product. Species cross-reactivity: Human, mouse, rat, chicken and bovine. Other species not tested.

Aufreinigung: Protein G purified

Immunogen: GST fusion protein containing full-length human paxillin.

Type of Immunogen: Fusion protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects paxillin in RIPA lysates from 3T3 fibroblasts using 20 μg per lane for minigels. Immunoprecipitation: 4 μg per 500 μg of cell lysate. Immunocytochemistry:5-10 μg/mL (gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7 % paraformaldehyde for 5 minutes).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Paxillin

Target: Paxillin (PXN)

Andere Bezeichnung: PXN / Paxillin

Hintergrund: Name/Gene ID: PXN

Synonyms: PXN, Paxillin

Gen-ID: 5829

UniProt: P49023

Pathways: MAPK Signalweg, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling