E2F1 Antikörper (AA 1-89, AA 342-386)
Kurzübersicht für E2F1 Antikörper (AA 1-89, AA 342-386) (ABIN210874)
Target
Alle E2F1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 1-89, AA 342-386
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Spezifität
- Recognizes human E2F-1 at 55kD. Species cross-reactivity: Xenopus.
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Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%) Rat, Elephant (94%) Opossum (89%) Chicken, Lizard (83%).
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Aufreinigung
- Protein G purified
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Immunogen
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GST fusion protein corresponding to full-length human E2F1 (Accession #NP_005216). Epitope: aa1-89 for clone KH20, aa342-386 for clone KH95.
Type of Immunogen: Fusion protein -
Isotyp
- IgG
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Applikationshinweise
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Approved: IP, WB
Usage: Western Blot Analysis: 0.5-2 μg/mL of antibody detected E2F-1 (~55kD) in a HeLa nuclear extract. Positive Antigen Control: HeLa nuclear extract. Use 20 μg per lane. Immunoprecipitation: 4 μg of a previous lot of antibody immunoprecipitated E2F-1 from HeLa nuclear extract. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeat freeze-thaw cycles.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- E2F1 (E2F Transcription Factor 1 (E2F1))
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Andere Bezeichnung
- E2F1
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Hintergrund
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Name/Gene ID: E2F1
Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3 -
Gen-ID
- 1869
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NCBI Accession
- NP_005216
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UniProt
- Q01094
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Pathways
- p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie
Target
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