Caspase 3 Antikörper

Produktnummer ABIN210737

Der Kaninchen Polyklonal anti-Caspase 3 Antikörper wird verwendet zum Nachweis von Caspase 3 in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für Caspase 3 Antikörper (ABIN210737)

Target: Caspase 3 (CASP3)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Caspase 3 Antikörper

Spezifität: Recognizes full-length Caspase 3 (Yama/Apopain) at 32kD and proteolytic fragments as demonstrated after in vitro cleavage of recombinant Caspase 3. Cross-reacts with human, mouse and rat.

Aufreinigung: Protein A purified

Immunogen: Human full-length Caspase 3 fusion protein containing a histidine-6 tag.

Type of Immunogen: Fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.5-2 μg/mL (will detect the 32kD precursor of Caspase 3 in RIPA lysates from non-stimulated human A431 cells, previous lots detected Caspase 3 in RIPA lysates of mouse 3T3 and rat PC12 cells). Use 20 μg per lane for minigels. Positive Antigen Control: non-stimulated A431 cell lysate. Western Blot Analysis: Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Caspase 3 (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.07 M Tris-glycine,  pH 7.4, 0.105 M sodium chloride, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: CASP3 / Caspase 3

Hintergrund: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gen-ID: 836

UniProt: P42574

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation