EGF Antikörper (Extracellular Domain)

Produktnummer ABIN205919

Produktdetails anti-EGF Antikörper

Target: EGF (Epidermal Growth Factor (EGF))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Spezifität: Recognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kD EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumor cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.

Aufreinigung: Purified

Immunogen: Recombinant human EGF.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Func (0.25 - 0.35 μg/mL), IHC, IHC-P (0.25 - 1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase. Histology Positive Control Tissue: Human skin ELISA: LS-C42950 may be used in an indirect ELISA system or as a capture antibody in a sandwich ELISA for human EGF in combination with LS-C42951 as a detection reagent.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water. For long term storage the addition of 0.09 % sodium azide is recommended. For functional studies do not add azide.

Konzentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4.

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF (EGF Produkte)

Hintergrund: Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor

Gen-ID: 1950

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation