CDKN2A Antikörper (C-Term)

Produktnummer ABIN1981278

Dieses Anti-CDKN2A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CDKN2A in WB, IF, IHC (p) und FACS. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für CDKN2A Antikörper (C-Term) (ABIN1981278)

Target: CDKN2A (Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4) (CDKN2A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDKN2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: RB19093

Produktdetails anti-CDKN2A Antikörper

Bindungsspezifität: AA 104-131, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This p16INK4a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the C-terminal region of human p16INK4a.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:10~50. WB: 1:2000. WB: 1:2000. IHC-P-Leica: 1:500. FC: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-CDKN2A Antikörper (ABIN1981278)

Han, Li, Zhang, Qin, Yang, Wang, Yuan, Xia: "Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells." in: International journal of oncology, Vol. 51, Issue 5, pp. 1439-1448, (2018) (PubMed).

Details zu CDKN2A

Target: CDKN2A (Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4) (CDKN2A))

Andere Bezeichnung: p16INK4a

Hintergrund: This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene, this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Molekulargewicht: 16533

Gen-ID: 1029

NCBI Accession: NP_000068, NP_001182061, NP_478104

UniProt: P42771

Pathways: Mitotic G1-G1/S Phases, Stem Cell Maintenance, Positive Regulation of Endopeptidase Activity, Autophagie, Positive Regulation of Response to DNA Damage Stimulus