ELK1 Antikörper (Ser389)

Produktnummer ABIN197160

Dieses Anti-ELK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ELK1 in WB, IF und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für ELK1 Antikörper (Ser389) (ABIN197160)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ELK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ELK1 Antikörper

Bindungsspezifität: Ser389

Spezifität: Elk-1 antibody detects endogenous levels of total Elk-1 protein.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic non-phosphopeptide derived from human Elk-1 around the phosphorylation site of serine 389 (P-R-SP-P-A).

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Andere Bezeichnung: ELK1

Hintergrund: The transcription factor Elk1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk1. Elk1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 (Ser383) is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.Synonyms: ETS domain-containing protein Elk-1, Elk-1

Gen-ID: 2002

NCBI Accession: NP_001107595

UniProt: P19419

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling