CDK2 Antikörper (pThr160)

Produktnummer ABIN196845

Der Kaninchen Polyklonal anti-CDK2 Antikörper wird verwendet zum Nachweis von CDK2 in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für CDK2 Antikörper (pThr160) (ABIN196845)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CDK2 Antikörper

Bindungsspezifität: pThr160

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. CDK2 (phospho-Thr160) antibody detects endogenous levels of CDK2 only when phosphorylated at threonine 160.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human CDK2 around the phosphorylation site of threonine 160 (T-Y-TP-H-E).

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1: 500approx. 1: 1000), ELISA (1: 4000) and Immunohistochemistry(1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Andere Bezeichnung: CDK2

Hintergrund: Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.Synonyms: Cell division protein kinase 2, Cyclin dependent kinase 2, p33 protein kinase

Gen-ID: 1017

NCBI Accession: NP_001789

UniProt: P24941

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA