Phospholipase C gamma 1 Antikörper (pTyr783)

Produktnummer ABIN196792

Produktdetails anti-Phospholipase C gamma 1 Antikörper

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Bindungsspezifität: pTyr783

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phospholipase C gamma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This antibody detects endogenous levels of PLC-gamma 1 only when phosphorylated at Tyrosine 783.

Aufreinigung: Affinity Chromatography using using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site

Immunogen: Peptide sequence around phosphorylation site of Tyrosine 783 (G-F-YP-V-E) derived from Human PLC-gamma-1 .

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1: 500approx. 1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Andere Bezeichnung: PLCG1 (PLCG1 Produkte)

Hintergrund: Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLC-gamma at Tyrosine 771, 783 and 1245. Phosphorylation of Tyrosine 783 leads to enzymatic activation of PLC-gamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1, PLC gamma1 Phospholipase C-II, PLC-148, PLC-II, PLC-gamma-1, PLC1, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-gamma-1

Gen-ID: 5335

NCBI Accession: NP_002651

UniProt: P19174

Pathways: RTK Signalweg, WNT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling