TRAF2 Antikörper (C-Term)

Produktnummer ABIN190911

Dieses Anti-TRAF2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von TRAF2 in WB, ELISA, IHC, IF und FACS. Geeignet für Human und Maus.

Kurzübersicht für TRAF2 Antikörper (C-Term) (ABIN190911)

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TRAF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-TRAF2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: TRAF2

Sequenz: KMEAKNSYVR DD

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KMEAKNSYVRDD, from the C Terminus of the protein sequence according to NP_066961.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Pancreas, Kidney and Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Ovary and Mouse Testis lysates and in lysates of cell lines HepG2 and Jurkat. Preliminary testing also showed a band at approx 50 kDa in U937, NIH3T3 and HEK293 cell lysates (calculated MW of 55.9 kDa, acco

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulums of HeLa and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended co

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TRAF2

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Andere Bezeichnung: TRAF2

Hintergrund: TRAF2, TNF receptor-associated factor 2 , MGC:45012, TRAP, TRAP3 , OTTHUMP00000064745, tumor necrosis factor type 2 receptor associated protein 3

Gen-ID: 7186, 22030

NCBI Accession: NP_066961

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response, S100 Proteine