XRCC6 Antikörper

Produktnummer ABIN187448

Der Maus Monoklonal anti-XRCC6 Antikörper wird verwendet zum Nachweis von XRCC6 in Proben von Human, Affe und Xenopus laevis. Er wurde validiert für WB, IF, IHC (p) und IP.

Kurzübersicht für XRCC6 Antikörper (ABIN187448)

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Reaktivität: Human, Affe, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser XRCC6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Klon: N3H10

Produktdetails anti-XRCC6 Antikörper

Spezifität: Reacts with the p70 Ku antigen subunit. Recognizes an epitope between aa 506-541 of p70. Species Reactivity: Human, monkey and Xenopus. Mouse and hamster (Western blots only). Does not react with bovine, rabbit or sea urchin. Other species not tested. Cellular Location: Nuclear.Positive Control: BT474, T47D, HEP-G-2 or HeLa cells. Tonsil.

Immunogen: Human placental extract designated as PSE1-PL.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunofluorescence Immunohistochemistry: (Formalin/paraffin) Use at a 1:400 dilution for 30 minutes at room temperature (RT). Staining of formalin-fixed tissues is improved by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling to RT for 20 min. Immunoprecipitation: (Native and denatured) Use at 2 µg/mg protein lysate. Use Protein A. Western blot: Use at 0.25-0.5 µg/mL for 2 hrs. at RT. The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 µg of monoclonal antibody at 200 µg/mL in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein A chromatography.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu XRCC6

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Andere Bezeichnung: XRCC6 / Ku70

Hintergrund: The Ku autoantigen is a heterodimer of 70 kDa (p70) and ~80 kDa (p80) proteins. The p70/p80 dimer is important for the function of a 460 kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53 and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis.

Pathways: DNA Reparatur