IFNA Antikörper (AA 24-172) (Biotin)

Produktnummer ABIN1862653

Produktdetails anti-IFNA Antikörper (Biotin)

Target: IFNA (Interferon alpha (IFNA))

Bindungsspezifität: AA 24-172

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IFNA Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequenz: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - CDLPQTH SLAHTRALRL LAQMRRISPF SCLDHRRDFG SPHEAFGGNQ VQKAQAMALV HEMLQQTFQL FSTEGSAAAW NESLLHQFYT GLDQQLRDLE ACVMQEAGLE GTPLLEEDSI RAVRKYFHRL TLYLQEKSYS PCAWEIVRAE VM

Spezifität: It has been selected for its ability to recognize IFNa in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Recombinant IFNa expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against IFNa conjugated to biotin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Kommentare:

Content: The quality control contains recombinant IFNa (Cys24~Met172) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Details zu IFNA

Target: IFNA (Interferon alpha (IFNA))

Andere Bezeichnung: Interferon Alpha (IFNA Produkte)

Synonyme: IFN-alphaO antikoerper, IFN-ALPHA-1 antikoerper, IFN1@ antikoerper, Ifa antikoerper, Ifa8 antikoerper, interferon alpha 16 antikoerper, interferon, alpha 1 antikoerper, interferon alpha antikoerper, interferon-alpha antikoerper, IFNA16 antikoerper, IFNA1 antikoerper, Ifna antikoerper, IFNA antikoerper

Pathways: JAK-STAT Signalweg, TLR Signalweg, Hepatitis C, Inflammasome