CHEK1 Antikörper (AA 194-446)

Produktnummer ABIN1858393

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CHEK1 in WB, IHC, ICC und IP. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für CHEK1 Antikörper (AA 194-446) (ABIN1858393)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-CHEK1 Antikörper

Bindungsspezifität: AA 194-446

Verwendungszweck: Polyclonal Antibody to Checkpoint Kinase 1 (CHEK1)

Spezifität: The antibody is a rabbit polyclonal antibody raised against CHEK1. It has been selected for its ability to recognize CHEK1 in immunohistochemical staining and western blotting.

Kreuzreaktivität: Human

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Checkpoint Kinase 1 (CHEK1) corresdonding to Cys194~Gly446 (Accession # O35280)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.5-2 μg/mL,1:220-880,Immunohistochemistry: 5-20 μg/mL,1:22-88,Immunocytochemistry: 5-20 μg/mL,1:22-88,Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.44 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: CHEK1

Hintergrund: CHK1, Cell cycle checkpoint kinase, Serine/threonine-protein kinase Chk1

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur