TRIM5 Antikörper (Internal Region)

Produktnummer ABIN185553

Dieses Anti-TRIM5-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von TRIM5 in ELISA und IHC. Geeignet für Human.

Kurzübersicht für TRIM5 Antikörper (Internal Region) (ABIN185553)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TRIM5 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-TRIM5 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: TRIM5 / RNF88 (alpha isoform)

Sequenz: CNIEKNENYQ PK

Spezifität: This antibody is expected to recognise isoform alpha (NP_149023.1) only.

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CNIEKNENYQPK, from the internal region of the protein sequence according to NP_149023.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments gave an approx 37 kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe g

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Andere Bezeichnung: TRIM5

Hintergrund: TRIM5, tripartite motif-containing 5 , HGNC:16276, RNF88, tripartite motif protein 5, tripartite motif protein TRIM5, TRIM5alpha, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha

Gen-ID: 85363

NCBI Accession: NP_149023

Pathways: Activation of Innate immune Response