Dynactin 1 Antikörper (C-Term)

Produktnummer ABIN184834

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Dynactin 1 in WB, ELISA, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für Dynactin 1 Antikörper (C-Term) (ABIN184834)

Target: Dynactin 1 (DCTN1)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Dynactin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Dynactin 1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Dynactin

Sequenz: QEQLHQLHSR LIS

Spezifität: This antibody is expected to recognise all reported isoforms.

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-7 μg/mL.

Western Blot: Approx. 150 kDa band observed in HeLa and MCF7 cell lysates, and in preliminary testing of HEK293 and Mouse Brain lysate (calculated MW of 142 kDa according to NP_004073.2). Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour at

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells and additionally the nucleus of U2OS cells.. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Dynactin 1 Antikörper (ABIN184834)

Bupha-Intr, Haizlip, Janssen: "Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H806-14, (2009) (PubMed).

Details zu Dynactin 1

Target: Dynactin 1 (DCTN1)

Andere Bezeichnung: DCTN1

Hintergrund: DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Gl

Gen-ID: 1639, 13191

NCBI Accession: NP_004073, NP_075408, NP_001128512, NP_001128513, NP_001177765, NP_001177766

Pathways: M Phase, ER-Nucleus Signaling