E2F1 Antikörper (AA 69-223)

Produktnummer ABIN1845545

Der Maus Monoklonal Anti-E2F1 Antikörper wurde für WB, ELISA, IHC und FACS validiert. Er ist geeignet, E2F1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für E2F1 Antikörper (AA 69-223) (ABIN1845545)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser E2F1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-E2F1 Antikörper

Bindungsspezifität: AA 69-223

Aufreinigung: Purified antibody

Immunogen: Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS with 0.05 % sodium azide and 0.5 % protein stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.

Details zu E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Andere Bezeichnung: E2F1

Hintergrund: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Molekulargewicht: 46.9 kDa

Gen-ID: 1869

UniProt: Q01094

Pathways: p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie