Leptin Antikörper (Biotin)

Produktnummer ABIN181605

Dieses Anti-Leptin-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Leptin in WB und EIA. Geeignet für Ratte.

Kurzübersicht für Leptin Antikörper (Biotin) (ABIN181605)

Target: Leptin (LEP)

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Leptin Antikörper (Biotin)

Spezifität: This antibody detects Leptin.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant Rat Leptin

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect Rat Leptin by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin. Sandwich: To detect Rat Leptin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-Rat Leptinas a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinantRat Leptin. Western Blot: To detect Rat Leptin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Leptin

Hintergrund: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

Gen-ID: 25608

NCBI Accession: NP_037208

UniProt: P50596

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process