Leptin Antikörper

Produktnummer ABIN181148

Der Maus Monoklonal anti-Leptin Antikörper wird verwendet zum Nachweis von Leptin in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für Leptin Antikörper (ABIN181148)

Target: Leptin (LEP)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Leptin Antikörper

Spezifität: This antibody detects Leptin.

Produktmerkmale: Synonyms: LEP, OB, OBS, Obesity factor, Obese protein

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Highly pure (>98%) recombinant Human Leptin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect recombinant human Leptin when used with Biotin conjugatedanti-Human Leptin antibody (cat. PP1041B) as the detection antibody at a concentration ofapproximately 0.5-1.0 μg/mL. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at aconcentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hLeptin is 2.0-4.0 ng/lane, undernon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Leptin

Hintergrund: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

Gen-ID: 3952

UniProt: P41159

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process