ZYX Antikörper (AA 352-357)

Produktnummer ABIN1742558

Produktdetails anti-ZYX Antikörper

Target: ZYX (Zyxin (ZYX))

Bindungsspezifität: AA 352-357

Reaktivität: Human, Maus, Ratte, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ZYX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Spezifität: Specific for zyxin.

Aufreinigung: purified IgG. Azide was added before lyophilization.

Immunogen: Recombinant human zyxin. Epitope TLKEVE (aa 352-357 of human zyxin)

Klon: 164D4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1 : 100 up to 1 : 2000 (AP staining)
ICC: 1 : 100
IHC: not tested yet

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-ZYX Antikörper (ABIN1742558)

Missirlis, Haraszti, Scheele, Wiegand, Diaz, Neubauer, Rechenmacher, Kessler, Spatz: "Substrate engagement of integrins α5β1 and αvβ3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin." in: Scientific reports, Vol. 6, pp. 23258, (2016) (PubMed).

Gadhari, Charnley, Marelli, Brugger, Chiquet: "Cell shape-dependent early responses of fibroblasts to cyclic strain." in: Biochimica et biophysica acta, Vol. 1833, Issue 12, pp. 3415-25, (2013) (PubMed).

Delaroche, Cantrelle, Subra, Van Heijenoort, Guittet, Jiao, Blanchoin, Chassaing, Lavielle, Auclair, Sagan: "Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 7712-21, (2010) (PubMed).

Low, Mukhina, Srinivas, Ng, Murata-Hori: "Domain analysis of alpha-actinin reveals new aspects of its association with F-actin during cytokinesis." in: Experimental cell research, Vol. 316, Issue 12, pp. 1925-34, (2010) (PubMed).

Mori, Nakagami, Koibuchi, Miura, Takami, Koriyama, Hayashi, Sabe, Mochizuki, Morishita, Kaneda: "Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis." in: Molecular biology of the cell, Vol. 20, Issue 13, pp. 3115-24, (2009) (PubMed).

Vollenbröker, George, Wolfgart, Saleem, Pavenstädt, Weide: "mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes." in: American journal of physiology. Renal physiology, Vol. 296, Issue 2, pp. F418-26, (2009) (PubMed).

Vervenne, Crombez, Delvaux, Janssens, Van de Ven, Petit: "Targeted disruption of the mouse Lipoma Preferred Partner gene." in: Biochemical and biophysical research communications, Vol. 379, Issue 2, pp. 368-73, (2009) (PubMed).

Guo, Wang: "Retrograde fluxes of focal adhesion proteins in response to cell migration and mechanical signals." in: Molecular biology of the cell, Vol. 18, Issue 11, pp. 4519-27, (2007) (PubMed).

Coutts, MacKenzie, Griffith, Black: "TES is a novel focal adhesion protein with a role in cell spreading." in: Journal of cell science, Vol. 116, Issue Pt 5, pp. 897-906, (2003) (PubMed).

Garvalov, Higgins, Sutherland, Zettl, Scaplehorn, Köcher, Piddini, Griffiths, Way: "The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions." in: The Journal of cell biology, Vol. 161, Issue 1, pp. 33-9, (2003) (PubMed).

Rottner, Krause, Gimona, Small, Wehland: "Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions." in: Molecular biology of the cell, Vol. 12, Issue 10, pp. 3103-13, (2001) (PubMed).

Details zu ZYX

Target: ZYX (Zyxin (ZYX))

Andere Bezeichnung: Zyxin (ZYX Produkte)