PCNA Antikörper (AA 53-196)

Produktnummer ABIN1724759

Der Maus Monoklonal Anti-PCNA-Antikörper wurde für WB, IHC, ELISA und FACS validiert. Er ist geeignet, PCNA in Proben von Human und Affe zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für PCNA Antikörper (AA 53-196) (ABIN1724759)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Klon: 7H4F8

Produktdetails anti-PCNA Antikörper

Bindungsspezifität: AA 53-196

Verwendungszweck: PCNA Antibody

Aufreinigung: Ascitic fluid

Immunogen: Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-PCNA Antikörper (ABIN1724759)

Takawa, Cho, Hayami, Toyokawa, Kogure, Yamane, Iwai, Maejima, Ueda, Masuda, Dohmae, Field, Tsunoda, Kobayashi, Akasu, Sugiyama, Ohnuma, Atomi, Ponder, Nakamura, Hamamoto: "Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating PCNA expression." in: Cancer research, Vol. 72, Issue 13, pp. 3217-27, (2012) (PubMed).

Zhao, Ho, Lo, Espejo, Bedford, Hung, Wang: "Interaction of proliferation cell nuclear antigen (PCNA) with c-Abl in cell proliferation and response to DNA damages in breast cancer." in: PLoS ONE, Vol. 7, Issue 1, pp. e29416, (2012) (PubMed).

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Molekulargewicht: 28.7 kDa

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie