CD74 Antikörper (AA 12-28)

Produktnummer ABIN1721492

Produktdetails anti-CD74 Antikörper

Target: CD74 (HLA-DR-gamma (CD74))

Bindungsspezifität: AA 12-28

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD74 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Spezifität: Reacts with human CD74.

Aufreinigung: Purified (protein G)

Immunogen: Synthetic peptide corresponding to the human CD74 invariant chain (aa 12-28) conjugated to KLH.

Klon: PIN1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
IHC (5 μg/mL) IP (12 μg/mL) WB (1 μg/mL) A doublet band of ~33-35 kDa is detected corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.

Positive control: PALA cell lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4, 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute in PBS or medium that is identical to that used in the assay system.

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one year at -20°C. Avoid repeated freezing and thawing.

Details zu CD74

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74 (CD74 Produkte)

Hintergrund: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain. CD74 functions mainly as an MHC class II chaperone which promotes ER exit of MHC class II molecules by directing them to endocytic compartments, preventing peptide binding in the ER, and contributing to peptide "editing" in the MHC class II compartment. CD74 has recently been shown to have a role as an accessory signaling molecule because of its high affinity binding to the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF).

HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, CD_antigen: CD74 Gene name : CD74

Gen-ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints