ANAPC10 Antikörper (AA 31-130) (Cy7)

Produktnummer ABIN1708179

Dieses Anti-ANAPC10-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ANAPC10 in WB, IF (cc) und IF (p). Geeignet für Ratte.

Kurzübersicht für ANAPC10 Antikörper (AA 31-130) (Cy7) (ABIN1708179)

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ANAPC10 Antikörper ist konjugiert mit Cy7

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-ANAPC10 Antikörper (Cy7)

Bindungsspezifität: AA 31-130

Kreuzreaktivität: Ratte

Homologie: Human,Mouse,Cow,Sheep,Pig,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human APC10

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ANAPC10

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Andere Bezeichnung: APC10

Hintergrund:

Synonyms: ANAPC 10, anapc10, Anaphase promoting complex 10, Anaphase promoting complex subunit 10, Anaphase-promoting complex subunit 10, Apc 10, APC10, APC10_HUMAN, Cyclosome subunit 10, DKFZP564L0562, Doc 1, Doc1, OTTHUMP00000220297, OTTHUMP00000220298, OTTHUMP00000220299, OTTHUMP00000220300, OTTHUMP00000220303.

Background: Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC10 contains a Doc1 homology domain, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases. APC10 binds to core APC subunits throughout the cell cycle. Specifically, APC10 binds to the C-terminus of CDC27/APC3. During mitosis, APC10 is localized in centrosomes and mitotic spindles. APC10 also localizes to kinetochores from prophase to anaphase, and to the midbody in telophase and cytokinesis.

Gen-ID: 10393

Pathways: M Phase