ATF2 Antikörper (pThr55) (AbBy Fluor® 647)

Produktnummer ABIN1699791

Dieses Anti-ATF2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ATF2 in WB, IF (cc) und IF (p). Geeignet für Maus und Ratte.

Kurzübersicht für ATF2 Antikörper (pThr55) (AbBy Fluor® 647) (ABIN1699791)

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATF2 Antikörper ist konjugiert mit AbBy Fluor® 647

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-ATF2 Antikörper (AbBy Fluor® 647)

Bindungsspezifität: pThr55

Kreuzreaktivität: Maus, Ratte

Homologie: Human

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Thr55

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Andere Bezeichnung: ATF2

Hintergrund:

Synonyms: ATF2 phospho T55, p-ATF2 phospho T55, p-ATF2 phospho T73 + T55, CREB 2, HB 16, Activating Transcription Factor 2, ATF 2, Atf-2, ATF2 protein, cAMP Response Element Binding Protein 2, cAMP response element binding protein CRE BP1, cAMP-dependent transcription factor ATF-2

Background: ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Gen-ID: 11909

Pathways: MAPK Signalweg, RTK Signalweg, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades