MAPKAP Kinase 2 Antikörper (Internal Region)

Produktnummer ABIN1590006

Dieses Anti-MAPKAP Kinase 2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von MAPKAP Kinase 2 in WB, IHC, ELISA und IP. Geeignet für Human und Maus.

Kurzübersicht für MAPKAP Kinase 2 Antikörper (Internal Region) (ABIN1590006)

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MAPKAP Kinase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Güteklasse: Verified

Produktdetails anti-MAPKAP Kinase 2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: MK2 / MAPKAPK2

Sequenz: SRVLKEDKER WED

Spezifität: This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45 kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0 kDa accord

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Andere Bezeichnung: MAPKAPK2

Hintergrund: MAPKAPK2, mitogen-activated protein kinase-activated protein kinase 2, MAPKAP-K2, MK-2, MK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2

Gen-ID: 9261, 17164, 289014

NCBI Accession: NP_004750, NP_116584

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades