MAPKAP Kinase 2 Antikörper (pThr334)

Produktnummer ABIN743693

Produktdetails anti-MAPKAP Kinase 2 Antikörper

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Bindungsspezifität: pThr334

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAPKAP Kinase 2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This phosphorylation site is homologous to Thr320 in Mouse and Rat. Due to the highly conserved nature of this region, this antibody may react with MAPKAPK3 when phosphorylated at Thr313 in Human, and Thr315 in Mouse and Rat based on an 80 % similarity.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Cow,Pig,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of Thr334

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Andere Bezeichnung: MAPKAPK2 (MAPKAPK2 Produkte)

Synonyme: mapkapk2 antikoerper, wu:fb30e10 antikoerper, wu:fc56c10 antikoerper, wu:fi03a01 antikoerper, MGC78852 antikoerper, MAPKAPK2 antikoerper, AA960234 antikoerper, MAPKAP-K2 antikoerper, MK-2 antikoerper, MK2 antikoerper, Rps6kc1 antikoerper, mitogen-activated protein kinase-activated protein kinase 2a antikoerper, mitogen-activated protein kinase-activated protein kinase 2 L homeolog antikoerper, mitogen-activated protein kinase-activated protein kinase 2 antikoerper, MAP kinase-activated protein kinase 2 antikoerper, mapkapk2a antikoerper, mapkapk2.L antikoerper, MAPKAPK2 antikoerper, Mapkapk2 antikoerper

Hintergrund:

Synonyms: MK2, MK-2, MAPKAP-K2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2

Background: Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.

Gen-ID: 9261

UniProt: P49137

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades