CHEK1 Antikörper (pSer317)

Produktnummer ABIN1531800

Der Kaninchen Polyklonal anti-CHEK1 Antikörper (ABIN1531800) detektiert spezifisch CHEK1 in WB, ELISA, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für CHEK1 Antikörper (pSer317) (ABIN1531800)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-CHEK1 Antikörper

Bindungsspezifität: AA 286-335, pSer317

Verwendungszweck: Chk1 (phospho Ser317) Antibody

Spezifität: Phospho-Chk1 (S317) Polyclonal Antibody detects endogenous levels of Chk1 protein only when phosphorylated at S317.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser317. AA range:286-335

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: Chk1

Hintergrund:

Synonyms: CHEK1, CHK1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1

Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011],

Gene Name: CHEK1

Protein Name: Serine/threonine-protein kinase Chk1

Molekulargewicht: 54 kDa

Gen-ID: 1111

OMIM: 603078

UniProt: O14757

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur