E2F1 Antikörper (pThr433)

Produktnummer ABIN1531270

Der Kaninchen Polyklonal anti-E2F1 Antikörper wird verwendet zum Nachweis von E2F1 in Proben von Human und Maus. Er wurde validiert für WB und ELISA.

Kurzübersicht für E2F1 Antikörper (pThr433) (ABIN1531270)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-E2F1 Antikörper

Bindungsspezifität: AA 388-437, pThr433

Verwendungszweck: E2F-1 (phospho Thr433) Antibody

Spezifität: Phospho-E2F-1 (T433) Polyclonal Antibody detects endogenous levels of E2F-1 protein only when phosphorylated at T433.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433. AA range:388-437

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Andere Bezeichnung: E2F1

Hintergrund:

Synonyms: E2F1, RBBP3, Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1

Background: The protein encoded byThis gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media

Gene Name: E2F1

Protein Name: Transcription factor E2F1

Molekulargewicht: 46 kDa

Gen-ID: 1869

OMIM: 189971

UniProt: Q01094

Pathways: p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie