CD25 Antikörper (PE)
Kurzübersicht für CD25 Antikörper (PE) (ABIN135178)
Target
Alle CD25 (IL2RA) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Spezifität
- Mouse CD25, Mr 55 kDa
-
Produktmerkmale
- Rat Anti-Mouse CD25-PE
-
Immunogen
- IL-2 dependent BALB/c helper T cell clone HT2
-
Isotyp
- IgG2b
-
-
-
-
Applikationshinweise
-
- Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Neut - Reported in literature
- Working Dilutions: Flow Cytometry FITC and BIOT conjugates 3 g/106 cells PE, APC, SPRD, PE/CY7, and AF700 conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
-
Kommentare
-
Inhibition of IL-2 or mitogen-induced T cell proliferation, In vitro blocking of IL-2 binding
-
Probenmenge
- 1 mL
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Konzentration
- 0.1 mg/mL
-
Buffer
- 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handhabung
-
Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. -
Lagerung
- 4 °C
-
Informationen zur Lagerung
- Store at 2-8°C
-
-
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
-
Andere Bezeichnung
- CD25
-
Hintergrund
- The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.
-
Pathways
- JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation
Target
-