RFP Antikörper (HRP)

Produktnummer ABIN129725

Hersteller Produkt- Nr.: 600-403-379

Der Kaninchen Polyklonal Anti-RFP-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, RFP in Proben von Discosoma zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für RFP Antikörper (HRP) (ABIN129725)

Target: RFP (Red Fluorescent Protein (RFP))

Reaktivität: Discosoma

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFP Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-RFP Antikörper (HRP)

Hersteller Produkt- Nr.: 600-403-379

Hersteller: Rockland

Verwendungszweck: RFP Antibody Peroxidase Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Produktmerkmale: Synonyms: rabbit anti-RFP antibody peroxidase conjugation, rabbit anti-Red Fluorescent Protein antibody peroxidase conjugation, HRP conjugated rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Aufreinigung:

Rabbit Anti-RFP Peroxidase Conjugated Antibody was prepared from monospecific rabbit antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Preadsorption: Pre-adsorbed

Immunogen:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Rabbit Anti-RFP Peroxidase Conjugated Antibody has been tested in ELISA and Western blot. It can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen or to detect RFP or RFP containing proteins on Western blots. References suggest that the antibody is also suitable for IHC. Optimal titers for applications should be determined by the researcher.

Western Blot Dilution: 1:2,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-RFP Antikörper (HRP) (ABIN129725)

Bohlen, El-Nahal, Sommer: "Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques." in: Frontiers in neuroanatomy, Vol. 13, pp. 84, (2019) (PubMed).

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: DsRed

Hintergrund: Background: Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19 % identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP.

UniProt: Q9U6Y8