RFP Antikörper (FITC)

Produktnummer ABIN129718

Hersteller Produkt- Nr.: 600-402-379

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RFP in WB, FM, DB und FLISA. Er zeigt eine Reaktivität gegenüber Discosoma.

Kurzübersicht für RFP Antikörper (FITC) (ABIN129718)

Target: RFP (Red Fluorescent Protein (RFP))

Reaktivität: Discosoma

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFP Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Produktdetails anti-RFP Antikörper (FITC)

Hersteller Produkt- Nr.: 600-402-379

Hersteller: Rockland

Verwendungszweck: RFP Antibody Fluorescein Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
   Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Produktmerkmale: Synonyms: rabbit anti-RFP antibody fluorescein conjugation, FITC conjugated rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Aufreinigung:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Preadsorption: Pre-adsorbed

Immunogen:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Labeling Ratio: 3.4

Anwendungsinformationen

Applikationshinweise:

Application Note: Polyclonal anti-RFP is designed to detect RFP and its variants. This fluorescein conjugated antibody has been tested by dot blot and can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. Optimal titers for applications should be determined by the researcher.

Western Blot Dilution: >1:10,000

FLISA Dilution: >1:20,000

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: DsRed

Hintergrund: Background: Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19 % identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP.

UniProt: Q9U6Y8