CHEK1 Antikörper (AA 305-330)

Produktnummer ABIN129667

Hersteller Produkt- Nr.: 600-401-906

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CHEK1 in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Saccharomyces cerevisiae.

Kurzübersicht für CHEK1 Antikörper (AA 305-330) (ABIN129667)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-CHEK1 Antikörper

Bindungsspezifität: AA 305-330

Hersteller Produkt- Nr.: 600-401-906

Hersteller: Rockland

Verwendungszweck: CHK1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against yeast CHK1 protein.

Produktmerkmale: Synonyms: rabbit anti-CHK1 Antibody, CHK-1, CHK 1, Serine/threonine-protein kinase CHK1, Checkpoint kinase 1

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 305-330 of S. cerevisiae CHK1.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 40-60 kDa in size corresponding to CHK1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.73 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: CHK1

Hintergrund: Background: CHK1 (also known as serine/threonine-protein kinase CHK1 and checkpoint kinase 1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present.  The kinase controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit. CHK1 is localized within the nucleus.

Gen-ID: 852577, 6319751

UniProt: P38147

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur