PCNA Antikörper (AA 50-75)

Produktnummer ABIN129658

Hersteller Produkt- Nr.: 600-401-887

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PCNA in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Microalgae (Isochrysis galbana).

Kurzübersicht für PCNA Antikörper (AA 50-75) (ABIN129658)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Microalgae (Isochrysis galbana)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-PCNA Antikörper

Bindungsspezifität: AA 50-75

Hersteller Produkt- Nr.: 600-401-887

Hersteller: Rockland

Verwendungszweck: PCNA Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against algal PCNA.

Produktmerkmale: Synonyms: rabbit anti-PCNA antibody, Proliferating cell nuclear antigen

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 50-75 of Isochrysis galbana PCNA.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~29 kDa in size corresponding to PCNA by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:5,000 - 1:32,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.42 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: Background: PCNA, or Proliferating Cell Nuclear Antigen, is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.  PCNA is a nuclear protein.

Gen-ID: 7715035

UniProt: Q9LD36

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie