PLK1 Antikörper (pThr210)
Kurzübersicht für PLK1 Antikörper (pThr210) (ABIN129581)
Target
Alle PLK1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- pThr210
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Hersteller Produkt- Nr.
- 600-401-446
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Hersteller
- Rockland
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Verwendungszweck
- PLK1 phospho T210 Antibody
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Kreuzreaktivität (Details)
- Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein.
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Produktmerkmale
- Synonyms: rabbit anti-PLK1 pT210 antibody, Serine/threonine-protein kinase PLK1, rabbit anti-polio like kinase 1 antibody, Serine/threonine protein kinase 13 antibody, PLK-1, PLK 1, STPK13
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Aufreinigung
- This antibody was affinity purified.
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Sterilität
- Sterile filtered
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Immunogen
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Immunogen: Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to an internal region near aa 200-225 of Human Polo-like kinase 1 (Plk1) protein.
Immunogen Type: Conjugated Peptide
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Isotyp
- IgG
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Applikationshinweise
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: This affinity-purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract. This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue.
Western Blot Dilution: 1:200 - 1:2,000
ELISA Dilution: 1:3,000 - 1:12,000
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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Modulation of key regulators of mitosis linked to chromosomal instability is an early event in ochratoxin A carcinogenicity." in: Carcinogenesis, Vol. 30, Issue 4, pp. 711-9, (2009) (PubMed).
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Modulation of key regulators of mitosis linked to chromosomal instability is an early event in ochratoxin A carcinogenicity." in: Carcinogenesis, Vol. 30, Issue 4, pp. 711-9, (2009) (PubMed).
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- PLK1 (Polo-Like Kinase 1 (PLK1))
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Andere Bezeichnung
- PLK1
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Hintergrund
- Background: Polo-like Kinase pT210 Antibody detects phosphorylated Plk1 protein. Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.
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Gen-ID
- 5347, 21359873
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UniProt
- P53350
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Pathways
- Zellzyklus, M Phase
Target
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