MCL-1 Antikörper

Produktnummer ABIN126984

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-MCL-1 Antikörper (ABIN126984) detektiert spezifisch MCL-1 in WB, IHC (p) und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund, Schwein, Affe, Kaninchen und Cat.

Kurzübersicht für MCL-1 Antikörper (ABIN126984)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reaktivität: Human, Maus, Ratte, Hund, Schwein, Affe, Kaninchen, Cat

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCL-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-MCL-1 Antikörper

Spezifität: This antibody detects Mcl-1.

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1

Anwendungsinformationen

Applikationshinweise: Western Blot (0.5-4 μg/mL). Immunoprecipitation (10-20 μg/mL). Immunohistochemistry (10-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.

Referenzen für anti-MCL-1 Antikörper (ABIN126984)

Wakatsuki, Tokunaga, Shibata, Araki: "GSK3B-mediated phosphorylation of MCL1 regulates axonal autophagy to promote Wallerian degeneration." in: The Journal of cell biology, Vol. 216, Issue 2, pp. 477-493, (2017) (PubMed).

Details zu MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Andere Bezeichnung: MCL1

Hintergrund: Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.Synonyms: BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, mcl1/EAT, r004

Gen-ID: 4170

NCBI Accession: NP_001184249

UniProt: Q07820

Pathways: MAPK Signalweg