MCL-1 Antikörper (Internal Region)

Produktnummer ABIN6657787

Produktdetails anti-MCL-1 Antikörper

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Bindungsspezifität: Internal Region

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCL-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity with Mcl-1 from other sources is unknown

Aufreinigung: Anti-Mcl-1 affinity purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence. .

Immunogen:

Immunogen: This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Rabbit Anti-Mcl-1 antibody is suitable for western blotting, immunoprecipitation, and ELISA. This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size. The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Referenzen für anti-MCL-1 Antikörper (ABIN6657787)

Dong, Vaux: "Glucocorticoids can induce BIM to trigger apoptosis in the absence of BAX and BAK1." in: Cell death & disease, Vol. 11, Issue 6, pp. 442, (2021) (PubMed).

Schubert, Rapp, Brauns-Schubert, Schlicher, Stock, Wissler, Weiß, Charvet, Borner, Maurer: "Requirement of GSK-3 for PUMA induction upon loss of pro-survival PI3K signaling." in: Cell death & disease, Vol. 9, Issue 5, pp. 470, (2018) (PubMed).

Guerra, Bird, Harvey, Dharia, Korshavn, Prew, Stegmaier, Walensky: "Precision Targeting of BFL-1/A1 and an ATM Co-dependency in Human Cancer." in: Cell reports, Vol. 24, Issue 13, pp. 3393-3403.e5, (2018) (PubMed).

Girnius, Davis: "JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins." in: Cell reports, Vol. 21, Issue 7, pp. 1910-1921, (2018) (PubMed).

Omari, Waters, Naranian, Kim, Perumalsamy, Chi, Greenblatt, Moley, Opferman, Jurisicova: "Mcl-1 is a key regulator of the ovarian reserve." in: Cell death & disease, Vol. 6, pp. e1755, (2016) (PubMed).

Schuler, Baumgartner, Klepsch, Chamson, Müller-Holzner, Watson, Oh, Hennighausen, Tymoszuk, Doppler, Villunger: "The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland." in: Cell death and differentiation, Vol. 23, Issue 1, pp. 41-51, (2016) (PubMed).

Doi, Liu, Gowda, Barth, Claxton, Amin, Loughran, Wang: "Maritoclax induces apoptosis in acute myeloid leukemia cells with elevated Mcl-1 expression." in: Cancer biology & therapy, Vol. 15, Issue 8, pp. 1077-86, (2015) (PubMed).

Rutherford, Childs, Ohotski, McGlynn, Riddick, MacFarlane, Tasker, Pyne, Pyne, Edwards, Palmer: "Regulation of cell survival by sphingosine-1-phosphate receptor S1P1 via reciprocal ERK-dependent suppression of Bim and PI-3-kinase/protein kinase C-mediated upregulation of Mcl-1." in: Cell death & disease, Vol. 4, pp. e927, (2014) (PubMed).

Perciavalle, Stewart, Koss, Lynch, Milasta, Bathina, Temirov, Cleland, Pelletier, Schuetz, Youle, Green, Opferman: "Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration." in: Nature cell biology, Vol. 14, Issue 6, pp. 575-83, (2012) (PubMed).

Details zu MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Andere Bezeichnung: Mcl-1 (MCL1 Produkte)

Synonyme: BCL2L3 antikoerper, EAT antikoerper, MCL1-ES antikoerper, MCL1L antikoerper, MCL1S antikoerper, Mcl-1 antikoerper, TM antikoerper, bcl2-L-3 antikoerper, mcl1/EAT antikoerper, AW556805 antikoerper, mcl-1 antikoerper, fb72f02 antikoerper, wu:fb70a08 antikoerper, wu:fb72f02 antikoerper, zfMLP1 antikoerper, zfMcl-1a antikoerper, zgc:109835 antikoerper, zgc:109763 antikoerper, E3 antikoerper, E3-2 antikoerper, RNE3 antikoerper, RNU107 antikoerper, SNORA63A antikoerper, elF-4AII antikoerper, MCL1, BCL2 family apoptosis regulator antikoerper, myeloid cell leukemia sequence 1 antikoerper, MCL1, BCL2 family apoptosis regulator a antikoerper, MCL1, BCL2 family apoptosis regulator b antikoerper, small nucleolar RNA, H/ACA box 63 antikoerper, MCL1 antikoerper, Mcl1 antikoerper, mcl1a antikoerper, mcl1b antikoerper, SNORA63 antikoerper

Hintergrund:

Synonyms: rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody

Background: Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.

Gene Name: Mcl1

Gen-ID: 17210

UniProt: P97287

Pathways: MAPK Signalweg