Caspase 1 Antikörper

Produktnummer ABIN126963

Der Kaninchen Polyklonal anti-Caspase 1 Antikörper (ABIN126963) detektiert spezifisch Caspase 1 in WB, IP und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Caspase 1 Antikörper (ABIN126963)

Target: Caspase 1 (CASP1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Produktdetails anti-Caspase 1 Antikörper

Spezifität: This antibody recognizes 45 kDa Procaspase-1 and the 20 kDa cleaved product.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 132 of Human Caspase-1.

Anwendungsinformationen

Applikationshinweise: Western Blot (0.5-4 μg/mL). Immunoprecipitation (4-8 μg/mL). Immunofluorescence (10-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA as stabilizer and 0.01 % Thimerosal as preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Details zu Caspase 1

Target: Caspase 1 (CASP1)

Andere Bezeichnung: Caspase-1

Hintergrund: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role down stream of Caspase 8 which is involved in Fas-mediated apoptosis.Synonyms: CASP-1, CASP1, ICE, IL1BC, IL1BCE, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45

Gen-ID: 834

NCBI Accession: NP_001214

UniProt: P29466

Pathways: Apoptose, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome