Caspase 3 Antikörper (active, Cleavage Site, N-Term)

Produktnummer ABIN126957

Dieses Anti-Caspase 3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Caspase 3 in WB und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für Caspase 3 Antikörper (active, Cleavage Site, N-Term) (ABIN126957)

Target: Caspase 3 (CASP3)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Caspase 3 Antikörper

Bindungsspezifität: active, Cleavage Site, N-Term

Spezifität: The antibody preferentially recognizes the p17 fragment of the active caspase-3.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5-4 μg/mL. Immunohistochemistry on paraffin sections: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase-3

Hintergrund: Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.Synonyms: Apopain, CASP-3, CASP3, CPP-32, CPP32, Cysteine protease CPP32, SCA-1, SCA1, SREBP cleavage activity 1, Yama protein

Gen-ID: 836

NCBI Accession: NP_004337

UniProt: P42574

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation