CD25 Antikörper (PE)

Produktnummer ABIN120430

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD25 in FACS. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für CD25 Antikörper (PE) (ABIN120430)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD25 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: OX-39

Produktdetails anti-CD25 Antikörper (PE)

Spezifität: Recognizes the alpha chain of rat CD25, otherwise know as IL-2 receptor alpha, a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (1).

Aufreinigung: Protein G purified immunoglobulin fraction

Immunogen: Con A stimulated Rat T cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Reconstitute with 1 mL distilled water.

Buffer: PBS pH 7.4 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Haltbarkeit: 6 months

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25 / IL2RA

Hintergrund: The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gen-ID: 25704

NCBI Accession: NP_037295

UniProt: P26897

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation