C5AR1 Antikörper (N-Term)

Produktnummer ABIN118843

Dieses Maus Monoklonal-Antikörper erkennt spezifisch C5AR1 in WB, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh) und Kaninchen.

Kurzübersicht für C5AR1 Antikörper (N-Term) (ABIN118843)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reaktivität: Human, Rind (Kuh), Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C5AR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: S5-1

Produktdetails anti-C5AR1 Antikörper

Bindungsspezifität: AA 1-31, N-Term

Spezifität: This antibody recognises the C5a receptor (C5aR) CD88. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine and Rabbit.
Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation

Immunogen: C5aR - peptide: Met1 - Asn31. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 5 x 10e5 cells in 100 μL. Immunohistochemistry on paraffin sections. Western Blot. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Andere Bezeichnung: CD88 / C5R1

Hintergrund: CD88 is predominantly expressed on cells of the myeloid lineage.Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1

Gen-ID: 728

NCBI Accession: NP_001727

UniProt: P21730

Pathways: Komplementsystem