PLK1 Antikörper (pThr210) (Alexa Fluor 488)

Produktnummer ABIN1177154

Der Maus Monoklonal Anti-PLK1-Antikörper wurde für ICS validiert. Er ist geeignet, PLK1 in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für PLK1 Antikörper (pThr210) (Alexa Fluor 488) (ABIN1177154)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLK1 Antikörper ist konjugiert mit Alexa Fluor 488

Applikation: Intracellular Staining (ICS)

Klon: K50-483

Produktdetails anti-PLK1 Antikörper (Alexa Fluor 488)

Bindungsspezifität: pThr210

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human PLK1 Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Referenzen für anti-PLK1 Antikörper (Alexa Fluor 488) (ABIN1177154)

Peter, Gleixner, Cerny-Reiterer, Herrmann, Winter, Hadzijusufovic, Ferenc, Schuch, Mirkina, Horny, Pickl, Müllauer, Willmann, Valent: "Polo-like kinase-1 as a novel target in neoplastic mast cells: demonstration of growth-inhibitory effects of small interfering RNA and the Polo-like kinase-1 targeting drug BI 2536." in: Haematologica, Vol. 96, Issue 5, pp. 672-80, (2011) (PubMed).

Details zu PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Andere Bezeichnung: PLK1

Hintergrund: Polo-like kinase (PLK1) is a serine/threonine kinase with structural similarities to Drosophila's Polo kinase and the Cdc5p of Saccharomyces cerevisiae. Like its invertebrate counterparts, PLK1 activity is required for DNA synthesis and is regulated throughout the cell cycle. Furthermore, PLK1 is highly expressed in primary tumors. It associates with the mitotic spindle during mitosis suggesting that, in addition to its role during S phase, PLK1 may play a role during chromosome segregation. This is consistent with its potential role in cancer development. Threonine 210 (T210) is one of the major phoshorylation sites in activated PLK1 obtained from human mitotic cells. The K50-483 monoclonal antibody recognizes the phosphorylated T210 of human PLK1.

Pathways: Zellzyklus, M Phase